Studies on the physical and chemical mechanisms of separation of enantiomeric mixtures

Author: Bezhan Chankvetadze
Co-authors: Bezhan Chankvetadze, Antonina Mskhiladze, Lali Chankvetadze, Nino Takaishvili, Iza Matarashvili, Marina Karchkhadze
Keywords: Enantioseparations, High-performance liquid chromatography, Nano-liquid chromatography, Electrochromatography, Capillary electrophoresis
Annotation:

Studies on the physical and chemical mechanisms of separation of enantiomeric mixtures Bezhan Chankvetadze, Antonina Mskhiladze, Lali Chankvetadze, Nino Takaishvili, Iza Matarashvili, Marina Karchkhadze, Giorgi Jibuti, Khatuna Gogaladze, Ana Ghibradze, Maia Demetrashvili, Alexandre Dadianidze, Levan Mosiashvili Chair of Physical and Analytical Chemistry and the Institute of Physical and Analytical Chemistry, Iv. Javakhishvili Tbilisi State University, Chavchavadze Ave 1, 0179 Tbilisi, Georgia bezhan.chankvetadze@tsu.ge This presentation summarizes the publications made by our group in 2012 about the physical and chemical mechanisms of separation of enantiomeric mixtures. These studies are presented in 13 publications in scientific journals and numerous oral and poster presentations and seminars on scientific conferences. These studies are divided in following 3 groups: 1) Studies in the field of high-performance liquid chromatography; 2) Nano-chromatography and capillary electrochromatography and 3) Capillary electrophoresis. The studies of the first group are dealing with the method development and optimization for separation of enantiomeric mixtures based on novel polysaccharide-based chiral stationary phases by using statistical experimental design and multivariate optimization in normal-, reversed- and polar organic mobile phases, as well as the effect of a mobile phase, mobile phase additives and separation temperature on the enantiomer elution order. Together with this, the methods were developed for analysis of complex reaction mixtures of enantioselective homogenous aldol reactions. From this group of publications the especial attention deserves the work reporting the first application of core-shell type silica for preparation of polysaccharide-based chiral stationary phase. This is the first report in the literature on this topic. In the second group of the studies the applicability of novel polysaccharide based chiral stationary phases for analysis of chiral pesticides by using nano-liquid chromatography and capillary electrochromatography was shown, as well as the optimized methods were developed for screening of these materials. In addition, the application of core-shell silica based chiral stationary phases for separation of enantiomers in nano-liquid chromatography and capillary electrochromatography was shown for the first time. In the third group of our studies the separation mechanisms of enantiomers in capillary electrophoresis were studied by using nuclear magnetic resonance spectroscopy and the separation mechanisms in aqueous and non-aqueous capillary electrophoresis were compared.