Isolation and characterization of Salmonella and E.coli bacteriophages
Author: khatuna makalatiaCo-authors: I.Ediberidze,N.Ghudumidze, M.Merabishvili, I.Janashia, T.Dvalidze, N.Chanishvili
Keywords: Salmonella, E.coli, Bacteriophage, Screening, fRFLP tests
Annotation:
The present study aims isolation and selection of the phages active against Salmonella sp.and E.coli which would be then used for construction of theraputic or prophylactic bacteriophage mixtures applicable for humans and/or animals. Altogether 35 bacteriophage clones active against Salmonella sp.(30) and E.coli (5) have been isolated from 10 sewage samples. All newly isolated phage clones have been studied for the morphology of their negative plaques and virions and host ranges. The phages have been screened against 81 strains of different geographical origin related to S, typhimurium, S. enteritdis, S. agona, S.pullorum, S. gallinarum and S. cholera-suis. In total 7 phage clones related to Salmonella sp. and 4 phage clones related to E.coli have been tested using phage neutralization reaction and fRFLP tests.
Lecture files:
Isolation and characterization of Salmonella and E.coli bacteriophages [en]Salmonella - სა და E.coli - სპეციფიური ბაქტერიოფაგების გამოყოფა და მათი შესწავლა [ka]